Histone H2AX, Histone H2A.X, H2AX_HUMAN. UniProt Code History. P16104, Q4ZGJ7, Q6IAS5. Gene Synonyms. H2AX, H2a.x, H2afx, H2A/X. Supplier also known as. CST, Cell Signalling, New England Biolabs

I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I

Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). 2/14/2021 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (#9718) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=9718&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 I mmunohis t oc hemis t ry (P araf f in) 1: 480 2003-02-01 · Within minutes of the induction of DNA double-strand breaks in somatic cells, histone H2AX becomes phosphorylated at serine 139 and forms gamma-H2AX foci at the sites of damage. These foci then play a role in recruiting DNA repair and damage-response factors and changing chromatin structure to accurately repair the damaged DNA. Cells were cultured in DMEM Ham's F-12 containing 5% fetal calf serum (FCS) in a humidified atmosphere with 7% CO 2 at 37°C. Cells were checked before experimental use for mycoplasma contamination.

Gamma h2ax cell signaling

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Rabbit Polyclonal Anti-gamma H2AX [p Ser139] Antibody DNA Double-strand break marker cited in 26 publications. Validated: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Canine, and more. All lanes : Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655) at 1/1000 dilution Lane 1 : 3dpf zebrafish embryo whole cell extract Lane 2 : 3dpf zebrafish embryos treated with 8000 J/m2 UVC, whole cell extract Secondary All lanes : HRP-conjugated anti-rabbit IgG Developed using the ECL technique. Predicted band size: 15 kDa.

Monoclonal Antibody for studying H2AX (Ser139) phosphate in the Chromatin Regulation / Epigenetics research area. Phospho-Histone H2A.X (Ser139) (D7T2V) Mouse mAb | Cell Signaling Technology Go to your regional site?

ab11174 at 1/1000 staining human HeLa cells by ICC/IF. These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation.

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4).

Which aspects of the DDR are facilitated through this modification? Wash sections in dH 2 O two times for 5 min each. Wash sections in wash buffer for 5 min. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature. Remove blocking solution and add 100–400 µl primary antibody diluted in SignalStain ® Antibody Diluent ( #8112) to each section. H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3).

P16104, Q4ZGJ7, Q6IAS5. Gene Synonyms.
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Histone H2AX, Histone H2A.X, H2AX_HUMAN. UniProt Code History. P16104, Q4ZGJ7, Q6IAS5.

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Here we show that inactivation of the SWI/SNF complexes and downregulation of the catalytic core subunits of the complexes both result in inefficient DNA double-strand break (DSB) repair and increased DNA damage sensitivity as well as a large defect in H2AX phosphorylation (gamma-H2AX) and nuclear focus formation after DNA damage.

Utility of antiphosphorylated H2AX antibody (gamma-H2AX) in diagnosing metastatic renal cell carcinoma. Wasco MJ(1), Pu RT. Author information: (1)Department of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USA.

γH2AX foci formation at DSB sites occurs rapidly (within minutes) and is highly conserved from yeast to humans .

2010 Dec;95(12):1996-2004. doi: 10.3324/haematol.2010.026492.